Objective To investigate the changes of Genomic DNA methylation from whole bloodsamples in bipolar disorder patients. Methods Genome-wide DNA methylation levels in whole blood ob-tained from 10 bipolar disorder patients and 5 healthy controls were assayed by Infinium Human Methylation450 BeadChip (Illumina). The association of DNA methylation with bipolar disorder was analyzed by IMA of R project.Gene ontology and Pathway were analyzed by GCBI. Results Compared to healthy controls，DNA methylations of SLC6A3，ARNTL，MAGI2，FAM111A，ZNF578 and FAM196A genes were significantly upregulated，while DNA methylations of SMPD1，ZEB2，KCNQ5，FAM41C and RGS18 genes were significantly downregulated in bipolar disorder patients. Conclusions DNA methylation may be associated with bipolar disorder.