Objective To investigate the antidepressive effects of repetitive Transcranial Magnetic Stimulation（rTMS）combined with Sertraline （Ser）in rat model of Chronic Unpredictable Stress （CUS）rats as well as the influence on levels of 5-HIAA and 5-HT in cortex. Methods Totals of 40 adult male Sprague Dawley rats were randomly divided into five groups：Sham group，CUS group，CUS+rTMS group，CUS+S-er group and CUS+rTMS+Ser group. Rats in Sham group were treated with fake rTMS for consecutive 7 d after28 d normal breeding. Rats in CUS group and CUS+Ser group were treated with fake rTMS for consecutive 7 d after successful modeling. And rats in CUS+rTMS group and CUS+rTMS+Ser group were treated with rTMS with the same frequency and intensity of rTMS stimulation for consecutive 7 d after successful modeling. In addition，rats in CUS+Ser group and CUS+rTMS+Ser group received Ser ［10 mg/（kg·d）］intraperitoneally injection for7 d and other groups were injected intraperitoneally with the same dose of saline. Then the sugar preference test and open field test were performed to examine the behaviors. Meanwhile，at the end of the behavioral test，rats were sacrificed and the levels of 5-HIAA and 5-HT in cortex were measured by Enzyme Linked Immunosorbent Assay（ELISA）. Results （1）The sucrose preference percentage of the CUS group was significantly less than that of other groups （P＜0.05）. But there were no significant differences between CUS+rTMS group，CUS+Ser group and CUS+rTMS+Ser group （P＞0.05）.（2）The fraction of central distance and the time exploring in center of the CUS group was significantly reduced than that of other groups （P＜0.05）. And there was no significant difference between CUS+rTMS group and CUS+Ser group （P＞0.05）.（3）Compared with CUS group，the cortical levels of 5-HIAA in CUS+rTMS and CUS+rTMS+Ser group were significantly reduced （P＜0.01）. And there was signif-icant difference between CUS+Ser group and CUS+rTMS+Ser group （P＜ 0.05）. Meanwhile，the levels of 5-HT in CUS group was significantly reduced than that of other groups（P＜ 0.05）. Conclusions rTMS combined with Sertraline could ameliorate the depression-like behavior of CUS rats and this effect may be related with the up-regulation of 5-HT and down-regulating of 5-HIAA in cortex.