Objective To investigate the effect of miR-92b on proliferation， migration and invasion of U251 cells and its possible mechanism. Methods The differential expression of miRNA in glioma cell line U251 and human normal glial cell line HEB was screened by miRNA chip. miR-92b inhibitor was synthesized by chemical synthesis， the expression changes were verified by real-time PCR after transfection. Cell proliferation ability was detected by CCK-8 assay after transfection. Wound healing assay and Transwell assay were used to detected cell migration and invasion ability after transfection. Western blot was performed to detected U251 cell proliferation and migration related protein β-catenin， E-cadherin and IGFBP 3. Luciferase assay was detected to confirm wheather IGFBP-3 was the target gene of miR-92b. Results miRNA chip results showed that the level of miR-92b in U251 cell was significantly higher than that of HEB. Inhibited miR-92b significantly decreased U251 cell proliferation ability， migration and invasion（ P＜0.05）. Western blot results showed the expression of β-catenin was decreased（ P＜0.05） ， and the expression of E-cadherin was increased（ P＜0.05） after transfection miR-92b inhibitor. Luciferase assay showed that miR-92b could directly regulate IGFBP-3. Conclusions The expression of miR-92b in U251 cell was increased significantly. It may inhibit the U251 cell proliferation and migration through up-regulation the expression of IGFBP-3 protein.