Objective To explore the metabonomics characters of peripheral blood in withdrawal period of methamphetamine abusers. Methods A total of 40 abstinent methamphetamine abusers were recruited from Shanghai Gaojing Forced Isolation Detoxification Institute， and 40 health controls were recruited from social in July 2017. Gas chromatography- mass spectrometry technology was used to detect metabolic products in serum. Results Compared with heathy controls， the age and body mass index（ BMI） of abstinent methamphetamine abusers were not significant different（ P＞0.05）， but metabolic products in amino acid metabolic pathways and purine metabolic pathways were changed significantly（ FC＞1. 5， P＜0. 05）. Conclusions Methamphetamine abuse influences the metabolic process persistently， amino acid and purine metabolic disturbance still can be observed in the abstinent period.