Objective To investigate the expression of Nogo-A and its receptor NgR1 in the spinal dorsal horn in a rat model of tibial bone cancer pain. Methods A total of 60 female non-copulatory Sprague- Dawley（ SD） rats weighting 160-200 g were randomly divided into two groups（ n=30）：sham operation group（ Group S） and bone cancer pain group（ Group BCP）. Group BCP received injection of 5 μl Walker-256 breast cancer cells in pulp cavity of left side of tibia， and Group S received injection of 5 μl normal saline. The Mechanical Withdrawal Threshold（ MWT） of rats in each group were measured using von Frey filaments to assess pain behavioral changes on 1d before operation and 3， 7， 14 and 21 d after operation. The rats were then sacrificed and L 4～5 segments of the spinal cord were removed for determination for Nogo-A and its receptor NgR1 expression in spinal dorsal horn by Western Blot after pain threshold measurement on 7， 14 and 21 d after operation. Results Compared with Group S， the MWT was significantly decreased at 7 d MWT［ （3.63±1.61）g］， 14 d MWT ［（2.53±1.39）g］ and 21 d MWT［ （2.40±1.16）g］ in Group BCP（ P ＜ 0.05）. Compared with Group S， the expression of Nogo-A and its receptor NgR1 was significantly increased at 7， 14 and 21 d in Group BCP（ P＜0.05）. Conclusions Nogo-A and its receptor NgR1 in the spinal dorsal horn may involve in the development of tibial bone cancer pain.