Objectives To develop a virtual reality（ VR） tool to assess the cue-induced craving in methamphetamine（ MA） patients and to explore the mechanism of cue-reactivity in autonomic nerves system and cortex. Methods MA cue enriched and neutral VR environment is created with a panoramic camera and displayed in the VR headset. A total of 20 MA patients were recruited. They received VR exposure while being monitored for skin conductance， pulse rate and electroencephalogram（ EEG）. Craving was assessed by a self-report measure and information about substance abuse history were collected. Results Patients' skin conductance significantly increased from（ 7.44±3.76） μS to（ 7.87±3.97） μS in MA cue enriched VR environment compared with neutral exposure（ P=0.018）. The activation of dorsolateral prefrontal， frontopolar and orbital frontal cortex significantly decreased by 0.41，0.51 and 0.49 dBμV2/m2， respectively（ t=2.594， P＜ 0.05）. Conclusions The findings in this pilot study have revealed the patients' psychophysical and cortex reaction to the MA cue enriched VR environment， which might be related to the mesocorticolimbic reward circuit and the executive control circuit.