Objectives To analyze the relationship between the location of acute single cerebral infarction（ ASCI） and secondary restless leg syndrome（ SRLS）. Methods The data of 556 continuous patients with ASCI were collected retrospectively， including demographic characteristics， laboratory results， imaging data， coexisting diseases， clinical characteristics of ASCI. All the patients were divided into SRLS group and non-SRLS group. Comparing between the two groups， univariate analysis was used to explore the related factors of SRLS in patients with ASCI， and multivariate analysis was used to explore the independent related factors of SRLS in patients with ASCI. Results Of the 556 ASCI patients included， 26（ 4.68%） suffered SRLS. Univariate analysis revealed uric acid was associated with the morbidity of SRLS（ P＜0.05）， and the incidence of SRLS was higher in thalamic and pons ASCI patients. Multivariate analysis revealed that ASCI occurring in the location of thalamus（ OR=5.81，P＜ 0.05） and pons（ OR=2.65， P＜ 0.05） are independent predictors for SRLS. Conclusions In patients with ASCI， the location of infarction is associated with the morbidity of SRLS， and ASCI occurring in the thalamus and pons may be independent predictors for SRLS.