Objectives To investigate the possible role of interleukin-33( IL-33) in cognitive impairment caused by hypoperfusion of the carotid artery. Methods A total of 36 adult male Sprague-Dawley (SD) rats were randomly divided into 3 groups: sham operation( S) group, Lesion 2 weeks( L2w) group, and Lesion 4 weeks( L4w) group. The Morris water maze was used to test the learning and memory ability of each group. The expression of cleaved-caspase-3 in the hippocampus was tested by immunohistochemistry. The expression of IL-33 in the hippocampus was tested by Western-blot method. Results The differences in the water maze test performance and the expression of cleaved-caspase-3 and IL-33 were statistically significant between the three groups( F=64.201,P<0.05;F=233.558,P<0.05;F=51.498,P<0.05). Compared with the S group( 20.32±6.30)s, the escape latency of the L2w group( 46.67±9.49) s was significantly prolonged (t=-4.902,P < 0.05); compared with the L2w group, the escape latency of the L4w group( 81.51±14.67)s was significantly prolonged( t=-6.397,P< 0.05). Compared with the S group( 1.31±1.19), the expression of cleaved-caspase-3 in the hippocampus in the L2w group( 6.56±1.32) was significantly increased( t=-6.328, P<0.05); compared with the L2w group, the L4w group's( 18.78±5.83) expression of cleaved-caspase-3 in the hippocampus was significantly increased( t=-14.733,P<0.05). Compared with the S group( 0.26±0.02), the expression of IL-33 in hippocampus in the L2w group( 0.3±0.04) increased, but the results were not statistically significant( t=-1.530,P=0.147); compared with the L2w group, the L4w group's expression of IL-33 in the hippocampus( 0.49±0.06) was significantly increased( t=-7.924,P<0.05). Conclusions IL-33 may play a role in cognitive impairment caused by hypoperfusion of brain tissue.
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孟德龙 贾柯娟 李艳 张彬.颈动脉低灌注大鼠模型海马区IL-33 和cleaved-caspase-3的表达[J].神经疾病与精神卫生,2019,19(4): DOI :10.3969/j. issn.1009-6574.2019.04.018.