Objectives To observe the effect of Electroacupuncture（ EA） pretreatment in the prevention of post-traumatic stress disorder（ PTSD） and its underlying molecular mechanisms. Methods A total of 32 healthy male Sprague Dawley（ SD） rats were randomly divided into four groups after adjusting the environment CB1R and DAGLα in the prefrontal cortex of ESPS model rats. Conclusions Electroacupuncture pretreatment alleviated ESPS-induced anxiety-like behavior in rats， which may be related to increased expression of CB1R and DAGLα in the prefrontal cortex of the brain. Further studies are needed to clarify their relevance. for 1 week： Sham group， ESPS group， Sham+EA group and ESPS+EA group， with 8 rats in each group. Rats in Sham+EA group and ESPS+EA group received electroacupuncture stimulation（ Baihui， 1 mA， 2/15 Hz） for 30 minutes every day for 1 week. The other two groups received sham stimulation（ EA treatment without electricity） for 1 week. Rats in ESPS group and ESPS+EA group were then exposed to enhanced single prolonged stress（ ESPS）. Two weeks after ESPS， the behavior of each rat was observed in the open field test and elevatedplus maze test. The expression of endocannabinoid receptor type 1（ CB1R）， monoacylglycerol lipase（ MAGL） and diacylglycerol lipase（ DAGLα） were measured by Western blot. Results （1） ESPS treatment resulted in anxiety-like behavior in rats， reduced time and frequency of entering the open-field central area， and reduced frequency of entering the elevated cross arm. The expression levels of CB1R and DAGLα in the prefrontal cortex of rats in the ESPS group decreased， while the expression of MAGL did not change significantly. （2）Electroacupuncture pretreatment can ameliorate the anxiety-like behavior， and restore the expression of