Objective To explore the functional connection characteristics of left and right amygdala in first-episode of childhood and adolescent patients with depressive disorder by rest state functional magnetic resonance imaging， and to analyze the correlation between abnormal functional connectivity and depressive symptoms. Methods From Octorber 2015 to June 2019， a total of 21 outpatients and inpatients with firstepisode childhood and adolescent depressive disorder in Mental Health Center of the First Hospital of Hebei Medical University were selected as the patient group， and 20 healthy children and adolescents were recruited as the control group. All the subjects were scanned by resting-state functional magnetic resonance imaging（ fMRI）. The left and right amygdalas were used as the brain regions of interest for the whole brain functional connectivity analysis to compare the functional connectivity differences between the two groups. The 24-item Hamilton Depression Scale（ HAMD-24） was used to evaluate the severity of depressive symptoms of the patient group， so as to analyze the correlation between depressive symptoms and functional connectivity of left and right amygdala. Results Compared with the controls group， the functional connections between the right amygdala and right superior frontal gyrus， left superior frontal gyrus， right middle temporal gyrus were reduced（ t=-4.49，-4.54， -4.91， GRF corrected， all P ＜ 0.05）； the functional connections between the left amygdala and right precuneus， left dorsolateral prefrontal lobe were reduced（ t=-6.40，-5.51， GRF corrected， all P ＜ 0.05）. The functional connectivity between the right amygdala and the right middle temporal gyrus were negatively correlated with the HAMD-24 score（ r=-0.920，P＜0.05， Bonferroni corrected）. Conclusions The abnormal functional connectivity of bilateral amygdala at resting-state in first-episode of childhood and adolescent patients with depressive disorder and the decreased functional connectivity between the right amygdala and the right middle temporal gyrus are related to the severity of depressive symptoms.