Objective To explore the incidence of post-stroke fatigue and the related psychosocial factors， so as to lay a theoretical foundation for its prevention and treatment. Methods A total of 408 patients with stroke were investigated and analyzed by using the General Questionnaire， Fatigue Severity Scale （FSS）， Self-rating Depression Scale（ SDS）， Mini-Mental State Examination（ MMSE）， Simplified Coping Style Questionnaire（ SCSQ）， Family Care Index Together（ APGAR） and Social Support Revalued Scale（ SSRS）. Results The incidence of post-stroke fatigue among the 408 stroke patients was 64.5%. Single factor analysis showed that age， marital status， education， family function， depression， cognitive function， coping style and social support were related to post-stroke fatigue（ P ＜ 0.05）. Multiple factors regression analysis showed that family function（ OR=3.205，P=0.027）， depression（OR=0.200，P=0.001）， negative coping（ OR=0.106，P=0.006） and objective support（ OR=2.312，P=0.003） were predictors of post-stroke fatigue with statistical significance. Conclusions The incidence of post-stroke fatigue is high， good family function and more objective support can be used as protective factors for post-stroke fatigue. Depression and negative coping were risk factors for poststroke fatigue