Objective To investigate the association between earthquake stress during pregnancy and the methylation status of NR3C1 exon 1F promoter and the influence on glucolipid metabolism in adulthood. Methods From January 2014 to March 2015， 100 cases of earthquake stress during pregnancy were selected as the earthquake stress group， and 76 cases without earthquake stress were selected as the control group. According to the pregnant period of earthquake， the pregnant women were divided into early pregnancy exposure group（ 41 cases）， mid pregnancy exposure group（ 30 cases） and late pregnancy exposure group（ 29 cases）. The methylation degree of NR3C1 promoter region was detected by bisulfite sequencing， and the metabolic indexes such as height， weight， BMI， blood pressure， fasting blood glucose and blood lipid were collected. Rusults NR3C1 methylation level of prenatal earthquake exposure group was significantly higher than that of the control group， and the difference was statistically significant（ P ＜ 0.05）. After comparison of subgroups， the methylation degree of NR3C1 promoter in the second trimester exposure group was higher than that in the early and late pregnancy groups， and the differences were statistically significant（ P ＜ 0.01）. The BMI and total cholesterol of adult in earthquake stress group were higher than those in control group， the difference was statistically significant（ P＜ 0.01）. Pearson correlation analysis showed that the methylation degree of NR3C1 promoter region was positively correlated with total cholesterol（ r=0.256， P ＜ 0.05） in maternal earthquake stress group， while it was positively correlated with triglyceride and total cholesterol in the second trimester exposure group（ r=0.406， 0.378， P＜0.05）. Conclusions There was significant association between increased NR3C1 exon 1F methylation and glucolipid metabolism in adulthood with prenatal earthquake stress.