Objective To explore the association between single nucleotide polymorphism （SNP） of γ- aminobutyric acid （GABA） synthase glutamate decarboxylase 2 （GAD2） gene polymorphism loci and Tourette syndrome （TS）. Methods 101 children with TS who met the diagnostic criteria of the fourth edition of the American Diagnostic and Statistical Manual of mental disorders （DSM- Ⅳ） in the outpatient or inpatient department of Beijing Anding Hospital Affiliated to Capital Medical University from December 2013 to March 2017 were recruited. The peripheral blood of 101 children with TS and their biological parents were collected and DNA was extracted. The SNP loci was genotyped by TaqMan SNP genotyping assay. Using genetic analysis statistical software Plink 1.07 transmission disequilibrium test （TDT） was performed to evaluate whether TS is associated with the study site. Results TDT analysis showed there was no transmission disequilibrium between GAD2 gene SNP loci rs992990， rs3781108， rs2236418， rs7908975 polymorphism and TS （P＞ 0.05）. Conclusions There was no relationship between GAD2 gene SNP loci rs992990， rs3781108， rs2236418， rs7908975 and TS.