Objective To assess the efficacy and safety of normobaric oxygen therapy（ NBOT） in acute stroke by means of a systemically assess. Methods Randomized controlled trials（ RCTs） about normobaric oxygen therapy in acute stroke were searched from CNKI， Wanfang， VIP， CBM， Medline， EMBASE， Cochrane Library and other database in Chinese and English， and the time limitation was from the inception to May 1st， 2018. Two reviewers independently screened the quality of literature by Modified Jadad scale. Meta-analysis was performed using RevMan 5.3. Results A total of four studies included 8 905 stroke patients（ 5 808 patients in the trial group and 3 097 in the control group） were eligible and abstracted. Pooled analysis demonstrated that normobaric oxygen therapy in acute stroke was not associated with favorable functional outcomes（ modified Ranking Scale： 0-2）（RR=0.98， 95%CI=0.94-1.03，P=0.46）. With respect to safety outcomes， normobaric oxygen therapy in acute stroke was not associated with the risk of death（ RR=1.03，95%CI=0.91-1.16，P=0.67）. Conclusions This systemically assess does not support the NBOT improve long time outcome of the patients with either ischemic or hemorrhagic stroke. However， the possible benefits of earlier time window NBOT cannot be ruled out.