Objectives To observe the therapeutic effect of Jujube seeds extract on the sensitisation of rat nicotine behaviour， and to explore its neurochemical mechanism. Methods Thirty-six adult Sprague- Dawley（ SD） rats were divided into normal saline group（ group A）， single nicotine group（ group B）， nicotine behavioural sensitisation model group（ group C）， and jujube seed low-dose treatment group（ group D）， high dose treatment group（ group E） and high dose of jujube group（ group F）. Groups C， D and E were injected subcutaneously with nicotine［ 0.4 mg（/ kg·d）］ for 7 days. Groups A， B and F were injected with the same volume of normal saline for 7 days， and then discontinued for 3 days. During the withdrawal period， the D， E and F groups were administered with the jujube extract once a day respectively（ low dose group： 60 mg/kg， high dose group： 180 mg/kg）， and groups A， B and C were administered with equal volume of drinking water. On the third day， 1 hour after gastrointestinal administration， except for the A and F groups， the other groups of rats were given an activated dose of nicotine（ 0.4 mg/kg）. Immediately after using the rat spontaneous activity detection system to detect the spontaneous activity of rats within 60 min， then the ultra-high performance liquid chromatography（ HPLC） was used to detect the dopamine content in rat striatum. Results Compared with group A and group B， the spontaneous activity of rats in group C increased significantly， while the sensitisation of