Objectives To observe the short-term and long-term curative effect in the treatment of trigeminal neuralgia by nerve trunk injection for nerve damage treatment with mixture composed of adriamycin and anhydrous ethanol. Methods A total of 91 cases with trigeminal neuralgia patients were treated for nerve damage by transcutaneous puncture and injecting mixture composed of adriamycin and anhydrous ethanol into trigeminal nerve trunk. Pain visual analogue score（ VAS） and pain relief level were recorded at the time points of prior treatment， the treatment day and 7 days， 1 month， 3 months， 6 months， 1 year and 2 years after operation. Results The pain recurrence rate of 91 patients within one year was 5.49%（ 5/91）. VAS score after treatment was significantly lower than that before the treatment at each time point and lower than that on the treatment day （P ＜ 0.01）. The differences were all no statistical significance in VAS score by pairwise comparison between 3 months， 6 months， 1 year and 2 years after treatment（ P ＞ 0.05）. The significant efficiency was 49.45%， 83.52%， 95.60%， 94.51%， 96.55%， 93.02% and 86.57% respectively on the treatment day， and 7 days， 1 month， 3 months， 6 months， 1 year， 2 years after treatment（ P＜0.05）. The mean rank of pain relief level at 7 days，1 month，3 months， 6 months， 1 year and 2 years after treatment were significantly lower than that on the treatment day（ P ＜0.05）. The mean rank of each time point at 1 month， 3 months， 6 months， 1 year and 2 years after treatment was significantly lower than that at 7 days after treatment（ P ＜ 0.01）. There was no statistical significance in the mean rank in pain relief level of 1 month， 3 months， 6 months， 1 year and 2 years after treatment by pairwise comparison（ P ＞ 0.05）. No serious complications occurred in all patients. Conclusions The treatment of trigeminal neuralgia by nerve trunk injection for nerve damage treatment with mixture composed of adriamycin and anhydrous ethanol is easy to operate and has no serious complications with good short-term and long-term effects.