Abstract:Objective To explore the relationship between different categories of internet addiction and depressive symptoms among university students using latent profile analysis and analyze the mediating effect of cognitive emotion regulation strategies between the two. Methods Random sampling was used to select 889 university students in five grades from four colleges and universities in Xinxiang City in January 2023 for the study. The General Demographic Information Questionnaire， internet Addiction Questionnaire， Cognitive Emotion Regulation Questionnaire （CERQ）， and Patient Health Questionnaire-9 （PHQ-9） were used to investigate internet addiction， depressive disorder， and cognitive emotion regulation strategies among university students. Mplus 8.3 was used to analyze the latent profile of internet addiction. Pearson correlation was used to analyze the correlation between internet addiction， cognitive emotion regulation strategies， and depressive disorder among university students. PROCESS procedure of SPSS 27.0 was used to test for the mediating role played in the relationship between internet addiction categories and depressive disorder and cognitive emotion regulation strategies. Results A total of 889 questionnaires were distributed and 802 were recovered， with an effective recovery rate of 90.21%. Among 802 university students， the total score of internet Addiction Questionnaire， adaptive cognitive emotion regulation strategy dimension score， non-adaptive cognitive emotion regulation strategy dimension score in CERQ， and PHQ-9 score were （44.33±13.03）， （66.20±11.48）， （42.71±9.51）， and （5.98±4.51）， respectively. Pearson correlation analysis showed that， internet addiction， adaptive cognitive emotion regulation strategies， non-adaptive cognitive emotion regulation strategies and depressive disorder see were all correlated， with statistically significant differences （r=-0.091-0.486； all P ＜ 0.01）. Latent profile analysis was conducted using each entry of the internet Addiction Questionnaire as an analytical indicator， and the number of categories was gradually increased starting from one latent category. A total of five category models were fitted， with model 3 being the best model. Naming each category based on the characteristics of the entries' responses， the results showed that there were three latent categories of university students' internet addiction， which were normal internet use group （36.3%）， low-level internet addiction group （43.3%）， and highlevel internet addiction group （20.3%）. Using normal internet use group as the reference group， both low-level internet addiction group and high-level internet addiction group positively predicted depressive disorder （β=0.33， P ＜ 0.001； β=0.75， P ＜ 0.001）. There was a mediating effect for both adaptive cognitive emotion regulation strategies and nonadaptive cognitive emotion regulation strategies between high-level internet addiction group and depressive disorder， with mediating effect values of 0.06 ［95%CI （0.016，0.120）］ and 0.39 ［95%CI （0.291， 0.491）］. There was a mediating effect of non-adaptive cognitive emotion regulation strategies between low-level internet addiction group and depressive disorder， with a mediating effect value of 0.22 ［95%CI （0.156，0.297）］. Conclusions There is a certain amount of internet addiction among university students. Between high-level internet addiction and depressive disorder， there are mediating effects of adaptive and nonadaptive cognitive emotion regulation strategies. Between low-level internet addiction and depressive disorder， there is a mediating effect of nonadaptive cognitive emotion regulation strategies.

Abstract:Objective To explore the effect of a certain dose of endotoxin， lipopolysaccharide （LPS） on skull repair in rats and the role of alendronate sodium （ALN） in improving skull repair. Methods Twenty SD male rats were randomly divided into blank， control， LPS and LPS+ALN groups to establish an animal model of LPS-induced and LPS-combined ALN for skull defect repair. Microscopic computed tomography （Micro-CT） was used to observe the skull repair in rats， enzyme-linked immunosorbent assay （ELSIA） was used to detect the levels of three inflammatory factors， namely interleukin-1β （IL-1β）， interleukin-6 （IL-6） and tumor necrosis factor-α （TNF-α）， in the blood of rats， and hematoxylin-eosin （HE） staining was utilized to observe the bone structure and cells. The status of osteoclasts at the skull defect was examined using tartrate resistant acid phosphatase （TRAP） staining of sections， and the mechanism of bone growth and bone resorption was investigated using immunohistochemistry. Results The results of the tested factors showed that， compared with blank group or control group， LPS caused abnormal expression of erythrocytes， hemoglobin， and platelets with a statistical difference （P＜ 0.05）， and there were statistically significant differences in inflammatory factors such as IL-1β （P=0.014）， IL-6 （P=0.049）， and TNF-α （P=0.006） after ALN administration. Compared with blank group， Micro-CT showed that significant bone resorption occurred in control group， LPS group， and LPS+ALN group， but significant intercranial resorption occurred in LPS group on day 2， while resorption was suppressed after ALN treatment. （3） Analyzing the pathological results， LPS led to an increase in osteoclasts， causing bone loss and bone structure defects， and significant bone resorption occurred in the skull， while less bone resorption occurred in LPS+ALN group， and the markers of osteoclast differentiation， osteocalcin and transforming growth factor-β， were least expressed at 46 days， with a higher rate of repair than in the other groups. Conclusions LPS immersion triggers bone resorption， which is detrimental to the proliferation and differentiation of rat skull osteoblasts， and ALN treatment effectively accelerates osteogenesis and inhibits the inflammatory bone resorption caused by LPS， thus improving the skull repair.

Abstract:Objective To explore the clinical efficacy of a neurosurgical stereotactic surgical robotic system for severe brain stem hemorrhage. Methods From March 2018 to December 2022， 52 patients with severe brainstem hemorrhage admitted to the Department of Neurosurgery of South Taihu Hospital Affiliated to Huzhou College were selected for the study. The patients were divided into control group （n=23） and study group （n=29） based on different treatment. Surgical treatment group was treated with stereotactic robot to perform brainstem hematoma removal by puncture and drainage or hematoma removal by puncture and drainage plus extracerebral ventricular drainage. Control group was treated with medication and/or extracerebral ventricular drainage. Postoperative 30-day mortality， severe disability rates， complications， and Modified Rankin Scale （mRS） scores were compared between the two groups. Cox survival analysis was used to verify the effects of group， gender， age， hematoma volume， pulmonary infection， and intracranial infection factors on the prognosis and survival time of patients with severe brainstem hemorrhage. Results There was no statistically significant difference in Glasgow Coma Scale （GCS） score， mean hematoma volume， and gender between the two groups at the time of admission （P ＞ 0.05）， and the difference in age was statistically significant （P ＜ 0.05）. Postoperative 30-day mortality in study group was 17.24% （5/29）， which was lower than that of 56.52% （13/23） in control group， and the difference was statistically significant （P ＜ 0.05）. The 90-day mRS score in study group was 4.0 （4.0， 5.0）， which was lower than that in control group， which was 6.0 （5.0， 6.0）， and the difference was statistically significant （P ＜ 0.05）. The postoperative severe disability + mortality in study group was 27.59% （8/29）， which was lower than that of 73.91% （17/23） in control group， and the difference was statistically significant （P＜ 0.05）. Cox survival analysis showed that patients in study group had a longer survival time than control group， and patients without lung infection had a better survival time than patients with lung infection. Conclusions Surgical robotic frameless stereotactic puncture for removal and drainage of severe brainstem hemorrhage is effective， and the method can be applied clinically to reduce mortality， but the rate of severe disability remains high.

Abstract:Within minutes following a traumatic brain injury， a pronounced inflammatory response occurs in the affected brain. The post-traumatic inflammatory response encompasses the release of pro-inflammatory and anti-inflammatory cytokines， regulation of neurotrophic factors， cellular migration， and phagocytosis. The neuroinflammatory process is implicated in the occurrence of secondary brain injury while also facilitating neurorepair. Various cytokines play a role in the inflammatory response after craniocerebral trauma and exhibit a significant correlation with the severity of the injury and the prognosis. This review analyzes the mechanisms and expression of different inflammatory factors following traumatic brain injury， as well as their utility as biomarkers for prognosis and treatment. The findings aim to identify suitable cytokine targets for inflammatory-targeted therapies to improve the outcomes for patients with traumatic brain injuries.

Abstract:Traumatic brain injury （TBI） is a change in brain function and brain tissue structure triggered by an external force. There is growing evidence that neuromodulation plays a positive role in the prognosis of neurological function in post-injury patients. This paper reviews the mechanism of action and therapeutic principles of neuromodulation in disorders of consciousness， with a view to providing new ideas for the clinical application of this technology.

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Abstract:Objective To explore the characteristics of brain network changes in different subregions of the dorsolateral prefrontal cortex （DLPFC） in patients with chronic insomnia disorder （CID） accompanied by depressive disorder. Methods A total of 102 CID patients recruited from outpatient clinics of the Chengdu Second People's Hospital and neighboring community hospitals from January 2021 to June 2022 with 38 good sleep controls （GSC） were selected for the study. All subjects underwent comprehensive neuropsychological testing and MRI. Pittsburgh Sleep Quality Index （PSQI）， Self-Rating Depression Scale （SDS）， and Self-Rating Anxiety Scale （SAS） were used to assess participants' sleep quality， depression， and anxiety. During data preprocessing， 12 participants were excluded （10 of them were CID patients and 2 were GSC）. Based on SDS scores， 92 patients with CID were categorized into CID with depressive disorder group （CID-D group， SDS scores ≥ 53， n=36） and CID with non-depressive disorder group （CID-ND group， SDS scores ＜ 53，n=56）. Fourteen subregions of the DLPFC ［bilateral A9/46d， inferior frontal joint cortex （IFJ）， A46， A9/46v， A8vl， A6vl， A10l］ were selected from the Brainnetome brain atlas for the construction of a functional connectivity network of the DLPFC subregions based on seed points. A voxel-based analysis of variance （ANOVA） was used to compare the differences in the brain networks of the subregions of the DLPFC among the three groups. Pearson correlation was used to analyze the correlation between brain network characteristics of DLPFC subregions and depressive disorder and insomnia. Results There were no statistically significant differences between the three groups in terms of gender， age， and years of education （all P＞ 0.05）. PSQI， SAS and SDS scores were higher in CID-D and CID-ND groups than in GSC group， and the differences were statistically significant （all P ＜ 0.05）. There was no statistically significant difference between CID-D and CID-ND groups in terms of PSQI scores and duration of disease （all P ＞ 0.05）. One-way ANOVA showed that the differences in FC changes in different subregions of DLPFC were statistically significant among the three groups （P ＜ 0.05）. Among the 14 DLPFC subregions， there were differences in brain networks in 10 DLPFC subregions （bilateral A9/46d， bilateral IFJ， R.A46， bilateral A9/46v， L.A8vl， and bilateral A6vl）， of which， the DLPFC subregion networks that were elevated in patients in both CID-D group and CID-ND group， compared with those in GSC group， included R.A9/46d with right temporal middle gyrus， R.A46 with left middle temporal gyrus， L.A9/46v with left inferior frontal gyrus and left middle frontal gyrus， and R.A9/46v with bilateral middle temporal gyrus and bilateral superior temporal gyrus. The network of DLPFC subregions that were reduced in both CID-D and CIDND group patients included L.A9/46d with bilateral talar cortex， R.A46 with left inferior frontal gyrus and left middle frontal gyrus. Further analysis showed that compared with CID-ND group， the network of elevated DLPFC subregions in CID-D group included L.IFJ with the right middle temporal gyrus and right superior temporal gyrus， R.IFJ with the left postcentral gyrus， L.A6vl with the right superior temporal gyrus and the right middle temporal gyrus， and the network of reduced DLFPC subregions included R.A9/46d with the left crustal nucleus， left caudate nucleus， and left thalamus， and L.A9/46v with left inferior frontal gyrus， left middle frontal gyrus， and L.A8vl with left caudate nucleus， left shell nucleus and R.A6vl with bilateral caudate nuclei. Correlation analysis between FC of altered DLPFC subregions and depressive symptoms in patients in CID group showed that R.A9/46d （r=-0.30，P=0.003） was negatively associated with left chiasmatic nucleus， left caudate nucleus， and left thalamus， L.A8vl （r=-0.35，P ＜ 0.001） was negatively associated with left caudate nucleus and left chiasmatic nucleus， and R.A6vl （r=-0.28， P=0.008） was negatively associated with FC values in the bilateral caudate nucleus and SDS scores. L.IFJ （r=0.33， P=0.001） was positively correlated with right middle temporal gyrus and right superior temporal gyrus， and R.IFJ （r=0.33， P=0.001） was positively correlated with left postcentral gyrus， and L.A6vl （r=0.39， P＜ 0.001） was positively correlated with FC value of right superior temporal gyrus and right middle temporal gyrus， as well as SDS scores. Conclusions FC changes in the DLPFC subregion in patients with CID suggest that insomnia involves abnormal connectivity within regions of attention， emotion regulation， and memory processing

Abstract:Objective To investigate the effect of inhibiting A Disintegrin and Metalloproteinase 10 （ADAM10） on apoptosis of Alzheimer's disease APPswe695 cells， a model of Alzheimer's disease， and to explore its underlying mechanism. Methods APPswe695 cells were treated with the ADAM10 inhibitor GI254023X at concentrations of 1， 5， 10， and 20 μmol/L， with PBS and Dimethyl sulfoxide （DMSO） serving as blank and negative controls， respectively. Cell viability was assessed using the Cell Counting Kit-8 （CCK-8） assay， and protein expression levels of the serine/threonine kinase （Akt）/forkhead box protein O3a （FOXO3a） signaling pathway and apoptosis-related proteins were measured by Western blot. Results The total protein expression levels of serine/ threonine protein kinase （Akt） and Forkhead Box O3a （FOXO3a） in the GI254023X group were （0.973 0±0.157 7） and （1.149 8±0.196 7）， respectively， showing no significant differences compared to the PBS and DMSO groups （P＞0.05）. However， the ratio of p-FOXO3a （S253）/FOXO3a after 24 h of treatment with 10 μmol/L GI254023X was （0.866 7±0.075 8）， significantly lower than those of the PBS （1.000 0±0.000 0） and DMSO （1.016 2±0.050 6） groups （P＜ 0.05）. Conversely， the ratio of p-Akt （S473）/Akt after 24 h of treatment with 10 μmol/L GI254023X was （1.180 6±0.150 2）， significantly higher than those of the PBS （1.000 0± 0.000 0） and DMSO （0.893 2±0.041 3） groups （P＜ 0.05）. Additionally， GI254023X significantly reduced the expression of the anti-apoptotic protein B-cell lymphoma-2 （Bcl-2） （P ＜ 0.05） and significantly increased the expression of the pro-apoptotic proteins caspase-3， caspase-9， and Bcl-2-associated X protein （Bax） （P＜ 0.05）Conclusions Treatment of APPswe695 cells with 10 μmol / L ADAM10 inhibitor GI254023X significantly inhibited cell viability and induced apoptosis of APPswe695 cells through the Akt / FOXO3a signaling pathway.

Abstract:The 11th revision of International Classification of Diseases （ICD-11） introduces annoveldiagnosticsystem for personality disorders， encompassing a global severity dimension and specific trait domains. This review synthesizes the global research advancements on this new diagnostic， encompassing the development of assessment tools， the correlation between the new diagnostic system and traditional categorical systems， and the clinical advantages observed. The aim is to provide Chinese mental health professionals with a comprehensive guide for understanding and implementing the ICD-11 personality disorder diagnostic system in clinical practice.

Abstract:CD147 belongs to the immunoglobulin superfamily and is a transmembrane glycoprotein that participates in normal metabolism and pathological processes of diseases by binding to multiple factors. Recent studies show that the expression of CD147 is significantly increased in glioma cells， which plays a crucial role in the proliferation， invasion， and metastasis of gliomas. In addition， CD147 enhances the glycolytic activity of glioma cells through the Warburg effect， which not only accelerates tumor growth but is also closely related to the formation of tumor treatment resistance. Therefore， CD147 holds promise as a potential target for glioma treatment. Based on this， this paper reviews the structure， function， and mechanism of CD147 in tumors， as well as its expression and impact in gliomas， in order to provide clinical reference.

Abstract:Attention training technique is an important component of metacognitive therapy， and the effectiveness has been fully validated. It can be used as an independent intervention to significantly reduce individuals' anxiety levels. This technology enhances an individual's attention control ability and improves attention flexibility. It can effectively interrupt the thinking patterns that lead to maladjustment， thereby achieving the goal of relieving anxiety. This article provides a review from three aspects： the origin， intervention， and mechanism of attention training technique， in order to provide reference for the treatment and further research of anxiety patients.

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